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Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative <t>FACS</t> plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.
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Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative <t>FACS</t> plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.
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Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative <t>FACS</t> plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.
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Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative FACS plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.

Journal: Journal of Traditional and Complementary Medicine

Article Title: Anti-inflammatory and polarization-modulating effects of Houttuynia cordata in LPS-stimulated RAW264.7 macrophages

doi: 10.1016/j.jtcme.2025.05.001

Figure Lengend Snippet: Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative FACS plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.

Article Snippet: After the cells were fixed with 4 % paraformaldehyde, phagocytic uptake was analyzed via a Fluorescence-activated cell sorting (FACS) flow cytometer (CytoFLEX S, Backman, USA).

Techniques: Expressing